Anti-Human DGCR8 Polyclonal Antibody (HP031014)

Name: Anti-Human DGCR8 Polyclonal Antibody
Brand: abinScience
SKU: HP031014
Price: 168 USD
Availability: InStock

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Aperçu

Numéro de catalogue

HP031014

Description

Anti-Human DGCR8 Polyclonal Antibody (HP031014) is a rabbit polyclonal antibody detecting DGCR8 in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human DGCR8 (Asp436-Lys752).

Cible

DGCRK6, Microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, DGCR8, C22orf12

Purification

Purified by antigen affinity column.

Numéro d'accès

Q8WYQ5

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Microprocessor complex subunit DGCR8 is a ~86 kDa protein. Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding.

1. Nguyen, TA. et al. (2015) Cell 161, 1374-87. PMID: 26027739

2. Kwon, SC. et al. (2016) Cell 164, 81-90. PMID: 26748718

3. Gregory, RI. et al. (2004) Nature 432, 235-40. PMID: 15531877

4. Han, J. et al. (2004) Genes & development 18, 3016-27. PMID: 15574589

5. Landthaler, M. et al. (2004) Current biology : CB 14, 2162-7. PMID: 15589161

6. Han, J. et al. (2006) Cell 125, 887-901. PMID: 16751099

7. Pauley, KM. et al. (2006) EMBO reports 7, 904-10. PMID: 16906129

8. Yeom, KH. et al. (2006) Nucleic acids research 34, 4622-9. PMID: 16963499

9. Faller, M. et al. (2007) Nature structural & molecular biology 14, 23-9. PMID: 17159994

10. Alarcón, CR. et al. (2015) Nature 519, 482-5. PMID: 25799998

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human DGCR8 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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