Anti-Human DGKA Polyclonal Antibody (HB083014)

Name: Anti-Human DGKA Polyclonal Antibody
Brand: abinScience
SKU: HB083014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HB083014

Description

Anti-Human DGKA Polyclonal Antibody (HB083014) is a rabbit polyclonal antibody detecting DGKA in ELISA, IHC, WB. Suitable for Human, Bovine, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human DGKA (Met1-Thr118).

Cible

Diacylglycerol kinase alpha, 80 kDa diacylglycerol kinase, DAGK1, DGKA, Diglyceride kinase alpha, DAG kinase alpha, DAGK, DGK-alpha

Purification

Purified by antigen affinity column.

Numéro d'accès

P23743

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Diacylglycerol kinase alpha (DGKA) is a ~82 kDa protein. Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol.

1. Epand, RM. et al. (2004) Biochemistry 43, 14778-83. PMID: 15544348

2. Schaap, D. et al. (1990) FEBS letters 275, 151-8. PMID: 2175712

3. Gellett, AM. et al. (2012) Biochemical and biophysical research communications 422, 758-63. PMID: 22627129

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human DGKA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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