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| カタログ番号 | HC395014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human DNM1 Polyclonal Antibody (HC395014) is a rabbit polyclonal antibody detecting DNM1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human DNM1 (Met1-Arg271). | ||||||||
| ターゲット | DNM1, DNM, Dynamin-1 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q05193 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Dynamin-1 (DNM1) is a ~97 kDa protein. Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission and participates in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis as well as rapid endocytosis (RE). Associates to the membrane, through lipid binding, and self-assembles into rings and stacks of interconnected rings through oligomerization to form a helical polymer around the vesicle membrane leading to constriction of invaginated coated pits around their necks. Self-assembly of the helical polymer induces membrane tubules narrowing until the polymer reaches a length sufficient to trigger GTP hydrolysis. Depending on the curvature imposed on the tubules, membrane detachment from the helical polymer upon GTP hydrolysis can cause spontaneous hemifission followed by complete fission. May play a role in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells through its activation by dephosphorylation via the signaling downstream of EGFR. 1. Soulet, F. et al. (2005) Molecular biology of the cell 16, 2058-67. PMID: 15703209 2. Chappie, JS. et al. (2010) Nature 465, 435-40. PMID: 20428113 3. Srinivasan, S. et al. (2018) PLoS biology 16, e2005377. PMID: 29668686 4. van der Bliek, AM. et al. (1993) The Journal of cell biology 122, 553-63. PMID: 8101525 5. Lin, HC. et al. (1996) The Journal of biological chemistry 271, 27979-82. PMID: 8910402 6. Muhlberg, AB. et al. (1997) The EMBO journal 16, 6676-83. PMID: 9362482 7. Kong, L. et al. (2018) Nature 560, 258-262. PMID: 30069048 8. Hinshaw, JE. et al. (1995) Nature 374, 190-2. PMID: 7877694 9. Scaife, R. et al. (1998) Biochemistry 37, 17673-9. PMID: 9922133 10. Bashkirov, PV. et al. (2008) Cell 135, 1276-86. PMID: 19084269 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-DNM1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse cerebellum lysate
Lane 2: Rat brain lysate
Lane 3: Rat cerebellum lysate
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