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Anti-Human DOT1L IP Recombinant Antibody (SAA0200) (HP484013)

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Anti-Human DOT1L IP Recombinant Antibody (SAA0200)
Anti-Human DOT1L IP Recombinant Antibody (SAA0200)
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  • 1mg
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Vista general
Número de catálogoHP484013
Descripción
Anti-Human DOT1L IP Recombinant Antibody (SAA0200) (HP484013) is a mouse monoclonal antibody detecting DOT1L in ELISA, IF, IP, IP-MS. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IF, IP, IP-MS
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG2a, kappa
ID de clonSAA0200
Objetivo DOT1-like protein, Lysine N-methyltransferase 4, DOT1L, Histone-lysine N-methyltransferase, H3 lysine-79 specific, KMT4, H3-K79-HMTase, KIAA1814, Histone H3-K79 methyltransferase
Nivel de endotoxinas < 10 EU/mg as determined by LAL method.
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso Q8TEK3
RRID Anti-Human DOT1L IP Antibody (SAA0200) (abinScience Cat# HP484013, RRID:AB_3729734)
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:2000-1:20000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo

Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1L) is a ~164 kDa protein. Histone methyltransferase that methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

1. Feng, Q. et al. (2002) Current biology : CB 12, 1052-8. PMID: 12123582
2. Frisbie, VS. et al. (2024) Nature communications 15, 2467. PMID: 38503750
3. Singh, PR. et al. (2023) Nature communications 14, 8497. PMID: 38129415
4. Min, J. et al. (2003) Cell 112, 711-23. PMID: 12628190
Nota For research use only.
Isotype Control Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)
Imágenes
  • Anti-Human DOT1L IP Recombinant Antibody (SAA0200)

    SDS-PAGE

    SDS-PAGE for Anti-Human DOT1L IP Antibody (SAA0200)

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human DOT1L IP Recombinant Antibody (SAA0200)

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