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Anti-Human DOT1L IP Recombinant Antibody (SAA0200) (HP484013)

Anti-Human DOT1L IP Recombinant Antibody (SAA0200)
Anti-Human DOT1L IP Recombinant Antibody (SAA0200)
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Aperçu
Numéro de catalogueHP484013
Description
Anti-Human DOT1L IP Recombinant Antibody (SAA0200) (HP484013) is a mouse monoclonal antibody detecting DOT1L in ELISA, IF, IP, IP-MS. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IF, IP, IP-MS
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG2a, kappa
ID de cloneSAA0200
Cible DOT1-like protein, Lysine N-methyltransferase 4, DOT1L, Histone-lysine N-methyltransferase, H3 lysine-79 specific, KMT4, H3-K79-HMTase, KIAA1814, Histone H3-K79 methyltransferase
Niveau d'endotoxines < 10 EU/mg as determined by LAL method.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès Q8TEK3
RRID Anti-Human DOT1L IP Antibody (SAA0200) (abinScience Cat# HP484013, RRID:AB_3729734)
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1L) is a ~164 kDa protein. Histone methyltransferase that methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

1. Feng, Q. et al. (2002) Current biology : CB 12, 1052-8. PMID: 12123582
2. Frisbie, VS. et al. (2024) Nature communications 15, 2467. PMID: 38503750
3. Singh, PR. et al. (2023) Nature communications 14, 8497. PMID: 38129415
4. Min, J. et al. (2003) Cell 112, 711-23. PMID: 12628190
Note For research use only.
Isotype Control Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)
Images
  • Anti-Human DOT1L IP Recombinant Antibody (SAA0200)

    SDS-PAGE

    SDS-PAGE for Anti-Human DOT1L IP Antibody (SAA0200)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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