Anti-Human Desmoglein-3 Recombinant Antibody (AK23) (HW575013)

Name: Anti-Human Desmoglein-3 Recombinant Antibody (AK23)
Brand: abinScience
SKU: HW575013
Price: 126 USD
Availability: InStock

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1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HW575013

Reactivité des espèces

Human

Applications

IP, IF, ELISA

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a, kappa

ID de clone

AK23

Applications testées

FCM

Cible

Desmoglein-3, 130 kDa pemphigus vulgaris antigen, PVA, Cadherin family member 6, DSG3, CDHF6

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P32926

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Desmoglein-3 (DSG3) is a ~107 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes. Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle. Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells. May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3.

1. Uttagomol, J. et al. (2019) International journal of molecular sciences 20. PMID: 31835537

2. Koch, PJ. et al. (1998) Journal of cell science 111 ( Pt 17), 2529-37. PMID: 9701552

3. Tsang, SM. et al. (2012) The Journal of pathology 227, 81-93. PMID: 22294297

4. Tsang, SM. et al. (2012) Experimental cell research 318, 2269-83. PMID: 22796473

5. Rötzer, V. et al. (2016) The Journal of investigative dermatology 136, 301-10. PMID: 26763450

Note

For research use only

Isotype Control

Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)

Images

SDS-PAGE

SDS-PAGE for Anti-Human DSG3 Antibody (AK23)
Flow cytometry

Flow-cytometry using anti-human Desmoglein 3 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Desmoglein 3 monoclonal antibody (Catalog # HW575013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SEC-HPLC

SEC-HPLC detection for Anti-Human DSG3 Antibody (AK23).

Reférences

Fiche technique

Anti-Human Desmoglein-3 Recombinant Antibody (AK23).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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