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Anti-Human DXO Polyclonal Antibody (HD149014)

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Aperçu
Numéro de catalogueHD149014
Description
Anti-Human DXO Polyclonal Antibody (HD149014) is a rabbit polyclonal antibody detecting DXO. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human DXO (Thr31-Lys396).
Cible 5'-3' exoribonuclease DXO, DOM3L, DOM3Z, DXO, DeNADding enzyme DXO, Decapping and exoribonuclease protein, Dom-3 homolog Z, EC:3.1.13.-, EC:3.6.1.-, NAD-capped RNA hydrolase DXO, NG6
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès O77932
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Decapping and exoribonuclease protein (DXO) is a ~44 kDa protein. Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA. The NAD-cap is present at the 5'-end of some RNAs and snoRNAs. In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Preferentially acts on NAD-capped transcripts in response to environmental stress. Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs and mediates their subsequent degradation.

1. Jiao, X. et al. (2017) Cell 168, 1015-1027.e10. PMID: 28283058
2. Grudzien-Nogalska, E. et al. (2019) Nature chemical biology 15, 575-582. PMID: 31101919
3. Picard-Jean, F. et al. (2018) PloS one 13, e0193804. PMID: 29601584
4. Doamekpor, SK. et al. (2020) Nucleic acids research 48, 6136-6148. PMID: 32374864
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human DXO Polyclonal Antibody

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