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Anti-Human E2F6 Polyclonal Antibody (HF932014)

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Aperçu
Numéro de catalogueHF932014
Description
Anti-Human E2F6 Polyclonal Antibody (HF932014) is a rabbit polyclonal antibody detecting E2F6 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human E2F6 (Pro62-Glu237).
Cible Transcription factor E2F6, E2F-6, E2F6
Purification Purified by antigen affinity column.
Numéro d'accès O75461
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Transcription factor E2F6 is a ~31 kDa protein. Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression.

1. Trimarchi, JM. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 2850-5. PMID: 9501179
2. Gaubatz, S. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 9190-5. PMID: 9689056
3. Cartwright, P. et al. (1998) Oncogene 17, 611-23. PMID: 9704927
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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