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Anti-Human ECD Polyclonal Antibody (HF615014)

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Anti-Human ECD Polyclonal Antibody
Anti-Human ECD Polyclonal Antibody
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Vista general
Número de catálogoHF615014
Descripción
Anti-Human ECD Polyclonal Antibody (HF615014) is a rabbit polyclonal antibody detecting ECD in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ECD (Met10-Asn406).
Objetivo Protein ecdysoneless homolog, Human suppressor of GCR two, hSGT1, ECD
Purificación Purified by antigen affinity column.
Número de acceso O95905
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Protein ecdysoneless homolog is a ~72 kDa protein. Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes.

1. Zhang, Y. et al. (2006) Cancer research 66, 7167-75. PMID: 16849563
2. Suh, HW. et al. (2013) Biochemical and biophysical research communications 438, 264-9. PMID: 23880345
3. Kim, JH. et al. (2010) Biological chemistry 391, 9-19. PMID: 19919181
4. Sato, T. et al. (1999) Molecular & general genetics : MGG 260, 535-40. PMID: 9928932
5. Kim, JH. et al. (2009) The Journal of biological chemistry 284, 26402-10. PMID: 19640839
6. Mir, RA. et al. (2015) Molecular and cellular biology 36, 886-99. PMID: 26711270
7. Claudius, AK. et al. (2014) PLoS genetics 10, e1004287. PMID: 24722212
8. Saleem, I. et al. (2021) Molecular and cellular biology 41, e0010321. PMID: 33941617
Nota For research use only.
Imágenes
  • Anti-Human ECD Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ECD polyclonal antibody at ECD on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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