中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HB230014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human ECHS1 Polyclonal Antibody (HB230014) is a rabbit polyclonal antibody detecting ECHS1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Pongo abelii, and Bovine.
Highlights
|
||||||||
| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ECHS1 (Ala28-Gln290). | ||||||||
| ターゲット | ECHS1 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P30084 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 別名 | Enoyl-CoA hydratase, mitochondrial, mECH, mECH1, 4.2.1.17, 5.3.3.8, Enoyl-CoA hydratase 1, ECHS1, Short-chain enoyl-CoA hydratase, SCEH, ECHS1 | ||||||||
| 背景 | Enoyl-CoA hydratase, mitochondrial (ECHS1) is a ~31 kDa protein. Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding (3S)-3hydroxyacyl-CoA species through addition of a water molecule to the double bond. Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16. Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate. Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation. 1. Peters, H. et al. (2014) Brain : a journal of neurology 137, 2903-8. PMID: 25125611 2. Yamada, K. et al. (2015) Journal of medical genetics 52, 691-8. PMID: 26251176 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-ECHS1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Caco2 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: PC-3 cell lysate
Lane 5: 293T cell lysate
Lane 6: Mouse heart lysate
Lane 7: Rat liver lysate
Lane 8: Rat heart lysate
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
