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Anti-Human EED IP Recombinant Antibody (SAA0245) (HF671013)

Anti-Human EED IP Recombinant Antibody (SAA0245)
Anti-Human EED IP Recombinant Antibody (SAA0245)
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  • 1mg
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Aperçu
Numéro de catalogueHF671013
Reactivité des espècesHuman
ApplicationsELISA, IF, IP, IP-MS
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG
ID de cloneSAA0245
Cible Polycomb protein EED, WAIT-1, EED, hEED, WD protein associating with integrin cytoplasmic tails 1, Embryonic ectoderm development protein
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès O75530
RRID Anti-Human EED IP Antibody (SAA0245) (abinScience Cat# HF671013, RRID:AB_3729779)
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Polycomb protein EED is a ~50 kDa protein. Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Note For research use only.
Images
  • Anti-Human EED IP Recombinant Antibody (SAA0245)

    SDS-PAGE

    SDS-PAGE for Anti-Human EED IP Antibody (SAA0245)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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