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Anti-Human EHD1 Polyclonal Antibody (HP095014)

Anti-Human EHD1 Polyclonal Antibody
Anti-Human EHD1 Polyclonal Antibody
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Vista general
Número de catálogoHP095014
Descripción
Anti-Human EHD1 Polyclonal Antibody (HP095014) is a rabbit polyclonal antibody detecting EHD1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human EHD1 (Thr20-Ser402).
Objetivo PAST, EHD1, PAST1, PAST homolog 1, EH domain-containing protein 1, Testilin, hPAST1
Purificación Purified by antigen affinity column.
Número de acceso Q9H4M9
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

EH domain-containing protein 1 (EHD1) is a ~60 kDa protein. ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Plays a role in myoblast fusion.

1. Cai, B. et al. (2013) The Journal of biological chemistry 288, 30172-30180. PMID: 24019528
2. Naslavsky, N. et al. (2004) Molecular biology of the cell 15, 2410-22. PMID: 15020713
3. George, M. et al. (2007) BMC cell biology 8, 3. PMID: 17233914
4. Rahajeng, J. et al. (2010) The Journal of biological chemistry 285, 31918-22. PMID: 20801876
5. Xie, S. et al. (2019) Journal of cell science 132. PMID: 31615969
6. Lu, Q. et al. (2015) Nature cell biology 17, 228-240. PMID: 25686250
Nota For research use only.
Imágenes
  • Anti-Human EHD1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-EHD1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse lung lysate
    Lane 2: Rat lung lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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