Anti-Human EHD1 Polyclonal Antibody (HP095014)

Name: Anti-Human EHD1 Polyclonal Antibody
Brand: abinScience
SKU: HP095014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HP095014

説明

Anti-Human EHD1 Polyclonal Antibody (HP095014) is a rabbit polyclonal antibody detecting EHD1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human EHD1 (Thr20-Ser402).

ターゲット

PAST, EHD1, PAST1, PAST homolog 1, EH domain-containing protein 1, Testilin, hPAST1

精製

Purified by antigen affinity column.

アクセッション番号

Q9H4M9

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

EH domain-containing protein 1 (EHD1) is a ~60 kDa protein. ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Plays a role in myoblast fusion.

1. Cai, B. et al. (2013) The Journal of biological chemistry 288, 30172-30180. PMID: 24019528

2. Naslavsky, N. et al. (2004) Molecular biology of the cell 15, 2410-22. PMID: 15020713

3. George, M. et al. (2007) BMC cell biology 8, 3. PMID: 17233914

4. Rahajeng, J. et al. (2010) The Journal of biological chemistry 285, 31918-22. PMID: 20801876

5. Xie, S. et al. (2019) Journal of cell science 132. PMID: 31615969

6. Lu, Q. et al. (2015) Nature cell biology 17, 228-240. PMID: 25686250

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-EHD1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse lung lysate
Lane 2: Rat lung lysate

参考文献

データシート

Anti-Human EHD1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human EHD1 Polyclonal Antibody (HP095014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ