Anti-Human EIF2B4 Polyclonal Antibody (HP770014)

Name: Anti-Human EIF2B4 Polyclonal Antibody
Brand: abinScience
SKU: HP770014
Price: 96 USD
Availability: InStock

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Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HP770014

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human EIF2B4 (Ser201-Gln523).

Objetivo

Translation initiation factor eIF-2B subunit delta, EIF2BD, EIF2B4, eIF-2B GDP-GTP exchange factor subunit delta

Purificación

Purified by antigen affinity column.

Número de acceso

Q9UI10

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Translation initiation factor eIF2B subunit delta (EIF2B4) is a ~57 kDa protein. Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.

1. Sekine, Y. et al. (2015) Science (New York, N.Y.) 348, 1027-30. PMID: 25858979

2. Kashiwagi, K. et al. (2016) Journal of structural and functional genomics 17, 33-8. PMID: 27023709

3. Kashiwagi, K. et al. (2019) Science (New York, N.Y.) 364, 495-499. PMID: 31048492

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-EIF2B4 polyclonal antibody at 1μg/ml on various samples.
Lane 1: RAW264.7 cell lysate
Lane 2: K562 cell lysate

Referencias

Datasheet

Anti-Human EIF2B4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo