Anti-Human EIF2B4 Polyclonal Antibody (HP770014)

Name: Anti-Human EIF2B4 Polyclonal Antibody
Brand: abinScience
SKU: HP770014
Price: 96 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HP770014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human EIF2B4 (Ser201-Gln523).

Cible

Translation initiation factor eIF-2B subunit delta, EIF2BD, EIF2B4, eIF-2B GDP-GTP exchange factor subunit delta

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9UI10

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Translation initiation factor eIF2B subunit delta (EIF2B4) is a ~57 kDa protein. Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.

1. Sekine, Y. et al. (2015) Science (New York, N.Y.) 348, 1027-30. PMID: 25858979

2. Kashiwagi, K. et al. (2016) Journal of structural and functional genomics 17, 33-8. PMID: 27023709

3. Kashiwagi, K. et al. (2019) Science (New York, N.Y.) 364, 495-499. PMID: 31048492

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EIF2B4 polyclonal antibody at 1μg/ml on various samples.
Lane 1: RAW264.7 cell lysate
Lane 2: K562 cell lysate

Reférences

Fiche technique

Anti-Human EIF2B4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai