

| Каталожный номер | HP770014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Реактивность видов | Human, Mouse, Rat | ||||||||
| Применения | ELISA, IHC, WB | ||||||||
| Хозяинский вид | Rabbit | ||||||||
| Клоональность | Polyclonal | ||||||||
| Изотип | IgG | ||||||||
| Иммуноген | E. coli - derived recombinant Human EIF2B4 (Ser201-Gln523). | ||||||||
| Цель | Translation initiation factor eIF-2B subunit delta, EIF2BD, EIF2B4, eIF-2B GDP-GTP exchange factor subunit delta | ||||||||
| Очистка | Purified by antigen affinity column. | ||||||||
| Номер доступа | Q9UI10 | ||||||||
| Форма | Liquid | ||||||||
| Буфер хранения | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
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| Информация об использовании продукта |
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| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Фон | Translation initiation factor eIF2B subunit delta (EIF2B4) is a ~57 kDa protein. Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed. 1. Sekine, Y. et al. (2015) Science (New York, N.Y.) 348, 1027-30. PMID: 25858979 2. Kashiwagi, K. et al. (2016) Journal of structural and functional genomics 17, 33-8. PMID: 27023709 3. Kashiwagi, K. et al. (2019) Science (New York, N.Y.) 364, 495-499. PMID: 31048492 | ||||||||
| Примечание | For research use only. |

Western blot analysis was performed using anti-EIF2B4 polyclonal antibody at 1μg/ml on various samples.
Lane 1: RAW264.7 cell lysate
Lane 2: K562 cell lysate
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