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| カタログ番号 | HT342014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human EIF3F Polyclonal Antibody (HT342014) is a rabbit polyclonal antibody detecting eIF3f in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human EIF3F (Gly89-Ala240). | ||||||||
| ターゲット | Eukaryotic translation initiation factor 3 subunit F, eIF3f, Deubiquitinating enzyme eIF3f, 3.4.19.12, Eukaryotic translation initiation factor 3 subunit 5, eIF-3-epsilon, eIF3 p47, EIF3F, EIF3S5 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O00303 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Eukaryotic translation initiation factor 3 subunit F (EIF3F) is a ~37 kDa protein. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. 1. Masutani, M. et al. (2007) The EMBO journal 26, 3373-83. PMID: 17581632 2. Lee, AS. et al. (2015) Nature 522, 111-4. PMID: 25849773 3. Lee, AS. et al. (2016) Nature 536, 96-9. PMID: 27462815 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-EIF3F polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: HepG2 cell lysate
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