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Anti-Human EIF4A1 Polyclonal Antibody (HX179014)

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Anti-Human EIF4A1 Polyclonal Antibody
Anti-Human EIF4A1 Polyclonal Antibody
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Aperçu
Numéro de catalogueHX179014
Description
Anti-Human EIF4A1 Polyclonal Antibody (HX179014) is a rabbit polyclonal antibody detecting EIF4A1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human EIF4A1 (Thr242-Ile406).
Cible Eukaryotic initiation factor 4A-I, eIF4A-I, eIF-4A-I, EIF4A, EIF4A1, DDX2A, ATP-dependent RNA helicase eIF4A-1,
Purification Purified by antigen affinity column.
Numéro d'accès P60842
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Eukaryotic initiation factor 4A-I (EIF4A1) is a ~46 kDa protein. ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth.

1. Wolf, A. et al. (2010) The Journal of cell biology 188, 463-71. PMID: 20156963
Note For research use only.
Images
  • Anti-Human EIF4A1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-EIF4A1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: PC12 cell lysate
    Lane 3: A431 cell lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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