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| カタログ番号 | HX179014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human EIF4A1 Polyclonal Antibody (HX179014) is a rabbit polyclonal antibody detecting EIF4A1 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human EIF4A1 (Thr242-Ile406). | ||||||||
| ターゲット | Eukaryotic initiation factor 4A-I, eIF4A-I, eIF-4A-I, EIF4A, EIF4A1, DDX2A, ATP-dependent RNA helicase eIF4A-1, | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P60842 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Eukaryotic initiation factor 4A-I (EIF4A1) is a ~46 kDa protein. ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth. 1. Wolf, A. et al. (2010) The Journal of cell biology 188, 463-71. PMID: 20156963 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-EIF4A1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC12 cell lysate
Lane 3: A431 cell lysate
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