Anti-Human EIF4A1 Polyclonal Antibody (HX179014)

Name: Anti-Human EIF4A1 Polyclonal Antibody
Brand: abinScience
SKU: HX179014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HX179014

Описание

Anti-Human EIF4A1 Polyclonal Antibody (HX179014) is a rabbit polyclonal antibody detecting EIF4A1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human EIF4A1 (Thr242-Ile406).

Цель

Eukaryotic initiation factor 4A-I, eIF4A-I, eIF-4A-I, EIF4A, EIF4A1, DDX2A, ATP-dependent RNA helicase eIF4A-1,

Очистка

Purified by antigen affinity column.

Номер доступа

P60842

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Eukaryotic initiation factor 4A-I (EIF4A1) is a ~46 kDa protein. ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth.

1. Wolf, A. et al. (2010) The Journal of cell biology 188, 463-71. PMID: 20156963

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-EIF4A1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC12 cell lysate
Lane 3: A431 cell lysate

Ссылки

Даташит

Anti-Human EIF4A1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-Human EIF4A1 Polyclonal Antibody (HX179014)

Обзор

Изображения

Ссылки

Даташит

SDS