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| カタログ番号 | HY024014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human EIF4E Polyclonal Antibody (HY024014) is a rabbit polyclonal antibody detecting EIF4E in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human EIF4E (Asn30-Val217). | ||||||||
| ターゲット | Eukaryotic translation initiation factor 4E, EIF4F, mRNA cap-binding protein, eIF4E, eIF-4E, eIF-4F 25 kDa subunit, EIF4EL1, EIF4E | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P06730 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Eukaryotic translation initiation factor 4E (EIF4E) is a ~25 kDa protein. Acts in the cytoplasm to initiate and regulate protein synthesis and is required in the nucleus for export of a subset of mRNAs from the nucleus to the cytoplasm which promotes processes such as RNA capping, processing and splicing. Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. This protein recognizes and binds the 7-methylguanosine (m7G)-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Together with EIF4G1, antagonizes the scanning promoted by EIF1-EIF4G1 and is required for TISU translation, a process where the TISU element recognition makes scanning unnecessary. In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm. 1. McKendrick, L. et al. (2001) European journal of biochemistry 268, 5375-85. PMID: 11606200 2. Yanagiya, A. et al. (2012) Molecular cell 46, 847-58. PMID: 22578813 3. Chen, CC. et al. (2012) FEBS letters 586, 2260-6. PMID: 22684010 4. Kamenska, A. et al. (2014) Nucleic acids research 42, 3298-313. PMID: 24335285 5. Haimov, O. et al. (2018) Molecular and cellular biology 38. PMID: 29987188 6. Tomoo, K. et al. (2005) Biochimica et biophysica acta 1753, 191-208. PMID: 16271312 | ||||||||
| 注意事項 | For research use only. |
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