Anti-Human EIF4E2 Polyclonal Antibody (HT124014)

Name: Anti-Human EIF4E2 Polyclonal Antibody
Brand: abinScience
SKU: HT124014
Price: 168 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

50ug
100ug

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HT124014

Описание

Anti-Human EIF4E2 Polyclonal Antibody (HT124014) is a rabbit polyclonal antibody detecting EIF4E2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human EIF4E2 (Glu53-Pro245).

Цель

Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, h4EHP, mRNA cap-binding protein type 3, EIF4E2, EIF4EL3

Очистка

Purified by antigen affinity column.

Номер доступа

O60573

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Eukaryotic translation initiation factor 4E type 2 (EIF4E2) is a ~28 kDa protein. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation. In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap. In P-bodies, component of a complex that promotes miRNA-mediated translational repression. Involved in virus-induced host response by mediating miRNA MIR34A-induced translational silencing which controls IFNB1 production by a negative feedback mechanism.

1. Rosettani, P. et al. (2007) Journal of molecular biology 368, 691-705. PMID: 17368478

2. Morita, M. et al. (2012) Molecular and cellular biology 32, 3585-93. PMID: 22751931

3. von Stechow, L. et al. (2015) Molecular and cellular biology 35, 1254-68. PMID: 25624349

4. Zhang, X. et al. (2021) Molecular cell 81, 1187-1199.e5. PMID: 33581076

5. Rom, E. et al. (1998) The Journal of biological chemistry 273, 13104-9. PMID: 9582349

6. Chapat, C. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 5425-5430. PMID: 28487484

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human EIF4E2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа