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Anti-Human EMC2 Polyclonal Antibody (HC225014)

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Vista general
Número de catálogoHC225014
Descripción
Anti-Human EMC2 Polyclonal Antibody (HC225014) is a rabbit polyclonal antibody detecting EMC2. Suitable for Rat, Mouse, and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human EMC2 (Met1-Ser297).
Objetivo EMC2, ER membrane protein complex subunit 2, KIAA0103, TPR repeat protein 35, TTC35, Tetratricopeptide repeat protein 35
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q15006
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

ER membrane protein complex subunit 2 (EMC2) is a ~34 kDa protein. Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors.

1. Guna, A. et al. (2018) Science (New York, N.Y.) 359, 470-473. PMID: 29242231
2. Shurtleff, MJ. et al. (2018) eLife 7. PMID: 29809151
3. Chitwood, PJ. et al. (2018) Cell 175, 1507-1519.e16. PMID: 30415835
4. Pleiner, T. et al. (2020) Science (New York, N.Y.) 369, 433-436. PMID: 32439656
5. O'Donnell, JP. et al. (2020) eLife 9. PMID: 32459176
6. Pleiner, T. et al. (2021) Molecular cell 81, 2693-2704.e12. PMID: 33964204
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human EMC2 Polyclonal Antibody

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