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Обзор
Каталожный номерHC225014
Описание
Anti-Human EMC2 Polyclonal Antibody (HC225014) is a rabbit polyclonal antibody detecting EMC2. Suitable for Rat, Mouse, and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human EMC2 (Met1-Ser297).
Цель EMC2, ER membrane protein complex subunit 2, KIAA0103, TPR repeat protein 35, TTC35, Tetratricopeptide repeat protein 35
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа Q15006
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Фон

ER membrane protein complex subunit 2 (EMC2) is a ~34 kDa protein. Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors.

1. Guna, A. et al. (2018) Science (New York, N.Y.) 359, 470-473. PMID: 29242231
2. Shurtleff, MJ. et al. (2018) eLife 7. PMID: 29809151
3. Chitwood, PJ. et al. (2018) Cell 175, 1507-1519.e16. PMID: 30415835
4. Pleiner, T. et al. (2020) Science (New York, N.Y.) 369, 433-436. PMID: 32439656
5. O'Donnell, JP. et al. (2020) eLife 9. PMID: 32459176
6. Pleiner, T. et al. (2021) Molecular cell 81, 2693-2704.e12. PMID: 33964204
Примечание For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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