中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HC488014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human ENDOG Polyclonal Antibody (HC488014) is a rabbit polyclonal antibody detecting ENDOG in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.
Highlights
|
||||||||
| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ENDOG (Ala49-Lys297). | ||||||||
| ターゲット | Endonuclease G mitochondrial, Endo G, EC:3.1.30.-, ENDOG | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q14249, P38447, O08600 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Endonuclease G, mitochondrial (ENDOG) is a ~32 kDa protein. Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA. The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs. Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs. Promotes conservative recombination when the DNA is 5hmC-modified. Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response. 1. Robertson, AB. et al. (2014) Nucleic acids research 42, 13280-93. PMID: 25355512 2. Wang, W. et al. (2021) Nature communications 12, 476. PMID: 33473107 3. Wiehe, RS. et al. (2018) Oncotarget 9, 18309-18326. PMID: 29719607 | ||||||||
| 注意事項 | For research use only |
Western blot analysis was performed using anti-ENDOG polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
