





| Numéro de catalogue | HB104307 |
|---|---|
| Reactivité des espèces | Human |
| Applications | ELISA, FCM, IF, IHC, IP, SPR, WB |
| Espèce hôte | Mouse |
| Clonalité | Monoclonal |
| Isotype | IgG2a, kappa |
| ID de clone | C52 |
| Applications testées | FCM |
| Cible | Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, EGP, EGP314, EPCAM, Ep-CAM, Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TACSTD1, TROP1, Tumor-associated calcium signal transducer 1, hEGP314 |
| Niveau d'endotoxines | < 10 EU/mg |
| Pureté | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Numéro d'accès | P16422 |
| Forme | Liquid |
| Tampon de stockage | 0.01M PBS pH 7.4. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Contexte | Epithelial cell adhesion molecule (CD326/EPCAM) is a ~34 kDa protein. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. |
| Note | For research use only. |

SDS-PAGE for Anti-Human EPCAM Antibody (C52)

Detects recombinant human CD326/EPCAM (Catalog No: HB104011) in indirect ELISA

Flow-cytometry using anti-human CD326 antibody. HT29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD326 monoclonal antibody (Catalog HB104307, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.




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