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Anti-Human EpCAM/TROP1 Recombinant Antibody (C52) (HB104307)

Anti-Human EpCAM/TROP1 Recombinant Antibody (C52)
Anti-Human EpCAM/TROP1 Recombinant Antibody (C52)
Anti-Human EpCAM/TROP1 Recombinant Antibody (C52)
Anti-Human EpCAM/TROP1 Recombinant Antibody (C52)
Anti-Human EpCAM/TROP1 Recombinant Antibody (C52)
Anti-Human EpCAM/TROP1 Recombinant Antibody (C52)
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Aperçu
Numéro de catalogueHB104307
Reactivité des espècesHuman
ApplicationsELISA, FCM, IF, IHC, IP, SPR, WB
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG2a, kappa
ID de cloneC52
Applications testées FCM
Cible Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, EGP, EGP314, EPCAM, Ep-CAM, Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TACSTD1, TROP1, Tumor-associated calcium signal transducer 1, hEGP314
Niveau d'endotoxines < 10 EU/mg
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P16422
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Epithelial cell adhesion molecule (CD326/EPCAM) is a ~34 kDa protein. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Note For research use only.
Images
  • Anti-Human EpCAM/TROP1 Recombinant Antibody (C52)

    SDS-PAGE

    SDS-PAGE for Anti-Human EPCAM Antibody (C52)

  • Anti-Human EpCAM/TROP1 Recombinant Antibody (C52)

    Bioactivity

    Detects recombinant human CD326/EPCAM (Catalog No: HB104011) in indirect ELISA

  • Anti-Human EpCAM/TROP1 Recombinant Antibody (C52)

    Flow cytometry

    Flow-cytometry using anti-human CD326 antibody. HT29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD326 monoclonal antibody (Catalog HB104307, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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