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Anti-Human ETF1 Polyclonal Antibody (HD118014)

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Vista general
Número de catálogoHD118014
Descripción
Anti-Human ETF1 Polyclonal Antibody (HD118014) is a rabbit polyclonal antibody detecting ETF1. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ETF1 (Met1-Ser257).
Objetivo ERF1, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Protein Cl1, RF1, SUP45L1, TB3-1, eRF1
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P62495
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

Eukaryotic peptide chain release factor subunit 1 (ETF1) is a ~49 kDa protein. Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons. The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA. Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.

1. Song, H. et al. (2000) Cell 100, 311-21. PMID: 10676813
2. Alkalaeva, EZ. et al. (2006) Cell 125, 1125-36. PMID: 16777602
3. Feng, T. et al. (2014) Molecular cell 53, 645-54. PMID: 24486019
4. Brown, A. et al. (2015) Nature 524, 493-496. PMID: 26245381
5. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242
6. Oltion, K. et al. (2023) Cell 186, 346-362.e17. PMID: 36638793
7. Frolova, L. et al. (1994) Nature 372, 701-3. PMID: 7990965
8. Yamashita, A. et al. (2009) Genes & development 23, 1091-105. PMID: 19417104
9. Wang, X. et al. (2019) Cell 176, 625-635.e14. PMID: 30682371
Nota For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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