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Anti-Human FLII Polyclonal Antibody (HC469014)

Anti-Human FLII Polyclonal Antibody
Anti-Human FLII Polyclonal Antibody
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Aperçu
Numéro de catalogueHC469014
Description
Anti-Human FLII Polyclonal Antibody (HC469014) is a rabbit polyclonal antibody detecting FLII in ELISA, IHC, WB. Suitable for Mus musculus and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human FLII (Leu896-Phe1176).
Cible FLII
Purification Purified by antigen affinity column.
Numéro d'accès Q13045
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsProtein flightless-1 homolog, FLII, FLIL
Contexte

Protein flightless-1 homolog (FLII) is a ~144 kDa protein. Is a regulator of actin polymerization, required for proper myofibril organization and regulation of the length of sarcomeric thin filaments. It also plays a role in the assembly of cardiomyocyte cell adhesion complexes. Regulates cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation. May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling.

1. Lee, YH. et al. (2004) Molecular and cellular biology 24, 2103-17. PMID: 14966289
Note For research use only.
Images
  • Anti-Human FLII Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-FLII polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: A549 cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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