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| カタログ番号 | HW347014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human FUT6 Polyclonal Antibody (HW347014) is a rabbit polyclonal antibody detecting FUT6 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human FUT6 (Arg35-Thr359). | ||||||||
| ターゲット | Fucosyltransferase 6, FUT6, Galactoside 3-L-fucosyltransferase, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase FUT6, FCT3A, FucT-VI, Fucosyltransferase VI, Fuc-TVI | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P51993 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase FUT6 is a ~41 kDa protein. Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to the N-acetyl glucosamine (GlcNAc) of a distal alpha2,3 sialylated lactosamine unit of a glycoprotein- or a glycolipid-linked sialopolylactosamines chain or of a distal or internal lactosamine unit of a neutral glycoprotein- or a glycolipid-linked polylactosamines chain through an alpha-1,3 glycosidic linkage and participates in surface expression of the sialyl Lewis X (sLe(x)), Lewis X (Le(x)) and non sialylated VIM2 determinants. Moreover transfers fucose to H-type 2 (Fucalpha1-2Galbeta1-4GlcNAc) chain acceptor substrates and participates in difucosylated sialyl Lewis x determinants. Also fucosylates a polylactosamine substrate having a 6 sulfate modification at the GlcNAc moiety and gives rise to sialyl and non-sialyl 6-sulfo lewis X. Does not have activity towards type 1 ((Galbeta1-3GlcNAc)) and H-type 1 chain (Fucalpha1-2Galbeta1-3GlcNAc) acceptors substrates. 1. Izawa, M. et al. (2000) Cancer research 60, 1410-6. PMID: 10728707 2. Weston, BW. et al. (1992) The Journal of biological chemistry 267, 24575-84. PMID: 1339443 3. Koszdin, KL. et al. (1992) Biochemical and biophysical research communications 187, 152-7. PMID: 1520296 4. Shetterly, S. et al. (2007) The Journal of biological chemistry 282, 24882-92. PMID: 17604274 5. Mondal, N. et al. (2018) The Journal of biological chemistry 293, 7300-7314. PMID: 29593094 6. Cameron, HS. et al. (1995) The Journal of biological chemistry 270, 20112-22. PMID: 7650030 7. De Vries, T. et al. (1997) Glycobiology 7, 921-7. PMID: 9363434 8. Borsig, L. et al. (1998) Glycobiology 8, 259-68. PMID: 9451035 | ||||||||
| 注意事項 | For research use only. |
HepG2 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting FUT6 (HW347014) at a concentration of 27 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of FUT6 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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