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Anti-Human G6PD Polyclonal Antibody
Anti-Human G6PD Polyclonal Antibody
Anti-Human G6PD Polyclonal Antibody
Anti-Human G6PD Polyclonal Antibody
Цена(USD):
Спецификация:
  • 20ug
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерHY124014
Описание
Anti-Human G6PD Polyclonal Antibody (HY124014) is a rabbit polyclonal antibody detecting G6PD in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human G6PD (Met1-Leu515).
Цель Glucose-6-phosphate 1-dehydrogenase, G6PD
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа P11413
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Glucose-6-phosphate 1-dehydrogenase (G6PD) is a ~59 kDa protein. Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. Also catalyzes the conversion of NAADPH, which is produced by enzymes such as DUOX1, DUOX2 and NOX5 from NAADP and promotes Ca(2+) signaling during T cell activation, back to NAADP.

1. Gu, F. et al. (2021) Science signaling 14, eabe3800. PMID: 34784249
Примечание For research use only.
Изображения
  • Anti-Human G6PD Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-G6PD polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: LO2 cell lysate
    Lane 3: A431 cell lysate
    Lane 4: A549 cell lysate

  • Anti-Human G6PD Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-G6PD polyclonal antibody at 1ug/mL on mouse hippocampus.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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