Anti-Human GABARAP Polyclonal Antibody (HF008014)

Name: Anti-Human GABARAP Polyclonal Antibody
Brand: abinScience
SKU: HF008014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Vista general

Número de catálogo

HF008014

Descripción

Anti-Human GABARAP Polyclonal Antibody (HF008014) is a rabbit polyclonal antibody detecting GABARAP in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cavia porcellus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human GABARAP (Met1-Leu117).

Objetivo

MM46, GABA(A) receptor-associated protein, GABARAP, Gamma-aminobutyric acid receptor-associated protein, FLC3B

Purificación

Purified by antigen affinity column.

Número de acceso

O95166

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Gamma-aminobutyric acid receptor-associated protein (GABARAP) is a ~13 kDa protein. Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction. Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation.

1. Wang, H. et al. (1999) Nature 397, 69-72. PMID: 9892355

2. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837

3. Behrends, C. et al. (2010) Nature 466, 68-76. PMID: 20562859

4. Colecchia, D. et al. (2012) Autophagy 8, 1724-40. PMID: 22948227

5. Chino, H. et al. (2019) Molecular cell 74, 909-921.e6. PMID: 31006538

6. Genau, HM. et al. (2015) Molecular cell 57, 995-1010. PMID: 25684205

7. Lee, JH. et al. (2005) Biotechnology letters 27, 623-8. PMID: 15977068

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human GABARAP Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

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