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Anti-Human Gal-1/LGALS1 Polyclonal Antibody (HY272014)

Anti-Human Gal-1/LGALS1 Polyclonal Antibody
Anti-Human Gal-1/LGALS1 Polyclonal Antibody
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Aperçu
Numéro de catalogueHY272014
Description
Anti-Human Gal-1/LGALS1 Polyclonal Antibody (HY272014) is a rabbit polyclonal antibody detecting LGALS1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Bubalus bubalis.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human Gal-1/LGALS1 (Met1-Asp135).
Cible HLBP14, Lactose-binding lectin 1, LGALS1, HBL, HPL, Beta-galactoside-binding lectin L-14-I, Gal-1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, Galaptin, Galectin-1, 14 kDa laminin-binding protein, 14 kDa lectin
Purification Purified by antigen affinity column.
Numéro d'accès P09382
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Galectin-1 (Gal-1) is a ~14 kDa protein. Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69.

1. de la Fuente, H. et al. (2014) Molecular and cellular biology 34, 2479-87. PMID: 24752896
Note For research use only.
Images
  • Anti-Human Gal-1/LGALS1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Galectin-1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: PC-12 cell lysate
    Lane 3: LO2 cell lysate
    Lane 4: U2OS cell lysate
    Lane 5: HCT116 cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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