Anti-Human GAPDH Polyclonal Antibody (HY030014)

Name: Anti-Human GAPDH Polyclonal Antibody
Brand: abinScience
SKU: HY030014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY030014

説明

Anti-Human GAPDH Polyclonal Antibody (HY030014) is a rabbit polyclonal antibody detecting GAPDH in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Cat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human GAPDH (Met1-Glu335).

ターゲット

GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase

エンドトキシンレベル

Please contact with the lab for this information.

精製

Purified by antigen affinity column.

アクセッション番号

P04406, P16858, P04797

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ~36 kDa protein. Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.

1. Tisdale, EJ. (2002) The Journal of biological chemistry 277, 3334-41. PMID: 11724794

2. Ercolani, L. et al. (1988) The Journal of biological chemistry 263, 15335-41. PMID: 3170585

3. Arif, A. et al. (2012) Molecular and cellular biology 32, 5046-55. PMID: 23071094

4. Gao, X. et al. (2013) Cell host & microbe 13, 87-99. PMID: 23332158

5. Gao, X. et al. (2016) The Journal of biological chemistry 291, 18232-8. PMID: 27387501

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-GAPDH polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: K562 cell lysate
Lane 5: PC-3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: HT-29 cell lysate

参考文献

データシート

Anti-Human GAPDH Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human GAPDH Polyclonal Antibody (HY030014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

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