Anti-Human GCK Polyclonal Antibody (HW504014)

Name: Anti-Human GCK Polyclonal Antibody
Brand: abinScience
SKU: HW504014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HW504014

Descripción

Anti-Human GCK Polyclonal Antibody (HW504014) is a rabbit polyclonal antibody detecting GCK in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human GCK (Met1-Gln465).

Objetivo

Hexokinase-4, Hexokinase type IV, HK4, HK IV, Glucokinase, Hexokinase-D, GCK

Purificación

Purified by antigen affinity column.

Número de acceso

P35557

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Hexokinase-4 (GCK) is a ~52 kDa protein. Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion.

1. Christesen, HB. et al. (2002) Diabetes 51, 1240-6. PMID: 11916951

2. Cuesta-Muñoz, AL. et al. (2004) Diabetes 53, 2164-8. PMID: 15277402

3. Heredia, VV. et al. (2006) The Journal of biological chemistry 281, 40201-7. PMID: 17082186

4. Estalella, I. et al. (2008) Journal of human genetics 53, 460-466. PMID: 18322640

5. Pal, P. et al. (2009) Biochemistry 48, 814-6. PMID: 19146401

6. Raimondo, A. et al. (2014) Human molecular genetics 23, 6432-40. PMID: 25015100

7. Xu, LZ. et al. (1995) Biochemistry 34, 6083-92. PMID: 7742312

8. Takeda, J. et al. (1993) The Journal of biological chemistry 268, 15200-4. PMID: 8325892

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human GCK Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo