Anti-Human GCK Polyclonal Antibody (HW504014)

Name: Anti-Human GCK Polyclonal Antibody
Brand: abinScience
SKU: HW504014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HW504014

Description

Anti-Human GCK Polyclonal Antibody (HW504014) is a rabbit polyclonal antibody detecting GCK in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human GCK (Met1-Gln465).

Cible

Hexokinase-4, Hexokinase type IV, HK4, HK IV, Glucokinase, Hexokinase-D, GCK

Purification

Purified by antigen affinity column.

Numéro d'accès

P35557

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Hexokinase-4 (GCK) is a ~52 kDa protein. Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion.

1. Christesen, HB. et al. (2002) Diabetes 51, 1240-6. PMID: 11916951

2. Cuesta-Muñoz, AL. et al. (2004) Diabetes 53, 2164-8. PMID: 15277402

3. Heredia, VV. et al. (2006) The Journal of biological chemistry 281, 40201-7. PMID: 17082186

4. Estalella, I. et al. (2008) Journal of human genetics 53, 460-466. PMID: 18322640

5. Pal, P. et al. (2009) Biochemistry 48, 814-6. PMID: 19146401

6. Raimondo, A. et al. (2014) Human molecular genetics 23, 6432-40. PMID: 25015100

7. Xu, LZ. et al. (1995) Biochemistry 34, 6083-92. PMID: 7742312

8. Takeda, J. et al. (1993) The Journal of biological chemistry 268, 15200-4. PMID: 8325892

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human GCK Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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