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| カタログ番号 | HW504014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human GCK Polyclonal Antibody (HW504014) is a rabbit polyclonal antibody detecting GCK in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human GCK (Met1-Gln465). | ||||||||
| ターゲット | Hexokinase-4, Hexokinase type IV, HK4, HK IV, Glucokinase, Hexokinase-D, GCK | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P35557 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Hexokinase-4 (GCK) is a ~52 kDa protein. Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion. 1. Christesen, HB. et al. (2002) Diabetes 51, 1240-6. PMID: 11916951 2. Cuesta-Muñoz, AL. et al. (2004) Diabetes 53, 2164-8. PMID: 15277402 3. Heredia, VV. et al. (2006) The Journal of biological chemistry 281, 40201-7. PMID: 17082186 4. Estalella, I. et al. (2008) Journal of human genetics 53, 460-466. PMID: 18322640 5. Pal, P. et al. (2009) Biochemistry 48, 814-6. PMID: 19146401 6. Raimondo, A. et al. (2014) Human molecular genetics 23, 6432-40. PMID: 25015100 7. Xu, LZ. et al. (1995) Biochemistry 34, 6083-92. PMID: 7742312 8. Takeda, J. et al. (1993) The Journal of biological chemistry 268, 15200-4. PMID: 8325892 | ||||||||
| 注意事項 | For research use only. |
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