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| カタログ番号 | HD546014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human GEN1 Polyclonal Antibody (HD546014) is a rabbit polyclonal antibody detecting GEN1. Suitable for Human.
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human GEN1 (Met1-Pro473). | ||||||||
| ターゲット | EC:3.1.-.-, Flap endonuclease GEN homolog 1, GEN1 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q17RS7 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 背景 | Flap endonuclease GEN homolog 1 (GEN1) is a ~102 kDa protein. Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction. 1. Ip, SC. et al. (2008) Nature 456, 357-61. PMID: 19020614 2. Lee, SH. et al. (2015) eLife 4. PMID: 26682650 3. Chan, YW. et al. (2015) Nucleic acids research 43, 10882-92. PMID: 26578604 4. Shah Punatar, R. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 443-450. PMID: 28049850 | ||||||||
| 注意事項 | For research use only |
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