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Обзор
Каталожный номерHD546014
Описание
Anti-Human GEN1 Polyclonal Antibody (HD546014) is a rabbit polyclonal antibody detecting GEN1. Suitable for Human.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human GEN1 (Met1-Pro473).
Цель EC:3.1.-.-, Flap endonuclease GEN homolog 1, GEN1
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа Q17RS7
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Фон

Flap endonuclease GEN homolog 1 (GEN1) is a ~102 kDa protein. Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction.

1. Ip, SC. et al. (2008) Nature 456, 357-61. PMID: 19020614
2. Lee, SH. et al. (2015) eLife 4. PMID: 26682650
3. Chan, YW. et al. (2015) Nucleic acids research 43, 10882-92. PMID: 26578604
4. Shah Punatar, R. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 443-450. PMID: 28049850
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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