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Anti-Human Glutathione hydrolase Polyclonal Antibody (HB811014)

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Vista general
Número de catálogoHB811014
Descripción
Anti-Human Glutathione hydrolase Polyclonal Antibody (HB811014) is a rabbit polyclonal antibody detecting Glutathione hydrolase 1 proenzyme in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human GGT1 (Ser122-Ser299).
Objetivo CD224, EC:2.3.2.2, EC:3.4.19.13, EC:3.4.19.14, GGT, GGT 1, GGT1, Gamma-glutamyltransferase 1, Gamma-glutamyltranspeptidase 1, Glutathione hydrolase 1 heavy chain, Glutathione hydrolase 1 light chain, Glutathione hydrolase 1 proenzyme, Leukotriene-C4 hydrolase
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P19440, P36268, A6NGU5
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Glutathione hydrolase 1 proenzyme is a ~61 kDa protein. Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates (such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate, MCTR1) and other gamma-glutamyl compounds (such as leukotriene C4, LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.

1. Castonguay, R. et al. (2007) Biochemistry 46, 12253-62. PMID: 17924658
2. Wickham, S. et al. (2011) Analytical biochemistry 414, 208-14. PMID: 21447318
3. Dalli, J. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, 12232-12237. PMID: 27791009
4. Ikeda, Y. et al. (1995) The Journal of biological chemistry 270, 22223-8. PMID: 7673200
5. Ikeda, Y. et al. (1995) The Journal of biological chemistry 270, 12471-5. PMID: 7759490
6. Ikeda, Y. et al. (1993) The Journal of biological chemistry 268, 3980-5. PMID: 8095045
7. Ikeda, Y. et al. (1996) Journal of biochemistry 119, 1166-70. PMID: 8827453
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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