Anti-Human GNAO1 Polyclonal Antibody (HY153014)

Name: Anti-Human GNAO1 Polyclonal Antibody
Brand: abinScience
SKU: HY153014
Price: 96 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HY153014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human GNAO1 (Met1-Tyr354).

Cible

Guanine nucleotide-binding protein G(o) subunit alpha, GNAO1

Purification

Purified by antigen affinity column.

Numéro d'accès

P09471

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Guanine nucleotide-binding protein G(o) subunit alpha (GNAO1) is a ~40 kDa protein. Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins.

1. García-Nafría, J. et al. (2018) Nature 558, 620-623. PMID: 29925951

2. Ping, YQ. et al. (2021) Nature 589, 620-626. PMID: 33408414

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GNAO1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse cerebellum lysate
Lane 2: Rat brain lysate
Lane 3: Rat cerebellum lysate

Reférences

Fiche technique

Anti-Human GNAO1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai