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Anti-Human GPC1 Recombinant Antibody (SAA0530) (HW614107)

Anti-Human GPC1 Recombinant Antibody (SAA0530)
Anti-Human GPC1 Recombinant Antibody (SAA0530)
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Especificación:
  • 50ug
  • 100ug
Número:
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Vista general
Número de catálogoHW614107
Reactividad de especiesHuman
AplicacionesELISA, FCM
Especie huéspedHuman
ClonalidadMonoclonal
IsotipoIgG1, kappa
ID de clonSAA0530
Objetivo Glypican-1, GPC1
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso P35052
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo

Glypican-1 (GPC1) is a ~61 kDa protein. Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.

Nota For research use only.
Imágenes
  • Anti-Human GPC1 Recombinant Antibody (SAA0530)

    Flow Cytometry

    Flow-cytometry using anti-human GPC1 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC1 antibody monoclonal antibody (Catalog # HW614107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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