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Anti-Human GPC1 Recombinant Antibody (SAA0530) (HW614107)

Anti-Human GPC1 Recombinant Antibody (SAA0530)
Anti-Human GPC1 Recombinant Antibody (SAA0530)
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概要
カタログ番号HW614107
種反応性Human
アプリケーションELISA, FCM
宿主種Human
クローン性Monoclonal
アイソタイプIgG1, kappa
クローンIDSAA0530
ターゲット Glypican-1, GPC1
エンドトキシンレベル Please contact with the lab for this information.
純度 >95% as determined by SDS-PAGE.
精製 Protein A/G purified from cell culture supernatant.
アクセッション番号 P35052
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 0.09% Sodium azide.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

安定性と保存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Glypican-1 (GPC1) is a ~61 kDa protein. Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.

注意事項 For research use only.
イメージ
  • Anti-Human GPC1 Recombinant Antibody (SAA0530)

    Flow Cytometry

    Flow-cytometry using anti-human GPC1 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC1 antibody monoclonal antibody (Catalog # HW614107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
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