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Anti-Human Glypican 3 Recombinant Antibody (SAA2001) (HW310043)

Anti-Human Glypican 3 Recombinant Antibody (SAA2001)
Anti-Human Glypican 3 Recombinant Antibody (SAA2001)
Anti-Human Glypican 3 Recombinant Antibody (SAA2001)
Anti-Human Glypican 3 Recombinant Antibody (SAA2001)
Цена(USD):
Спецификация:
  • 20ug
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерHW310043
Реактивность видовHuman
ПримененияELISA, WB
Хозяинский видHuman
КлоональностьMonoclonal
ИзотипIgG4, kappa
ID клонаSAA2001
Проверенные приложения WB
Цель GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P51654
RRID

Anti-Human GPC3 Antibody (SAA2001) (abinScience Cat# HW310043, RRID:AB_3727913)
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Форма Liquid
Буфер хранения 0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:2000-1:20000
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон

Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.

1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063
2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623
3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449
4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790
Примечание For research use only.
Isotype Control Human IgG4 (S228P) Recombinant Isotype Control Antibody (13R4) (HP298207)
Изображения
  • Anti-Human Glypican 3 Recombinant Antibody (SAA2001)

    SDS-PAGE

    SDS-PAGE for Anti-Human GPC3 Antibody (SAA2001)

  • Anti-Human Glypican 3 Recombinant Antibody (SAA2001)

    Western blot

    Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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