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Anti-Human GPLD1 Polyclonal Antibody (HX189014)

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Aperçu
Numéro de catalogueHX189014
Description
Anti-Human GPLD1 Polyclonal Antibody (HX189014) is a rabbit polyclonal antibody detecting GPLD1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human GPLD1 (Met496-Asp840).
Cible GPLD1, PIGPLD1, Phosphatidylinositol-glycan-specific phospholipase D, GPI-PLD, GPI-specific phospholipase D, Glycoprotein phospholipase D, PI-G PLD, Glycosyl-phosphatidylinositol-specific phospholipase D
Purification Purified by antigen affinity column.
Numéro d'accès P80108
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Phosphatidylinositol-glycan-specific phospholipase D (GPLD1) is a ~92 kDa protein. Hydrolyzes the inositol phosphate linkage of glycosylphosphatidylinositol (GPI)-anchored membrane proteins, thereby releasing them from the cell surface. Cleaves on the inositol side of the anchor, leaving the phosphate group attached to the membrane. This release of GPI-anchored proteins can modulate diverse biological processes, including the activation of signaling pathways.

1. Watanabe, K. et al. (2007) The Journal of biological chemistry 282, 31643-55. PMID: 17720976
2. Davitz, MA. et al. (1989) The Journal of biological chemistry 264, 13760-4. PMID: 2760042
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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