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Anti-Human GPR37 Polyclonal Antibody (HT211014)

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Anti-Human GPR37 Polyclonal Antibody
Anti-Human GPR37 Polyclonal Antibody
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Vista general
Número de catálogoHT211014
Descripción
Anti-Human GPR37 Polyclonal Antibody (HT211014) is a rabbit polyclonal antibody detecting GPR37 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human GPR37 (Arg401-Leu443).
Objetivo Endothelin B receptor-like protein 1, G-protein coupled receptor 37, Prosaposin receptor GPR37, Parkin-associated endothelin receptor-like receptor, GPR37, PAELR, ETBR-LP-1
Purificación Purified by antigen affinity column.
Número de acceso O15354
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Prosaposin receptor GPR37 is a ~67 kDa protein. G-protein-coupled receptor that plays a role in several physiological pathways such as resolution of inflammatory pain and oligodendrocyte differentiation. Acts as a receptor for several ligands including prosaposin, osteocalcin or neuroprotectin D1. Ligand binding induces endocytosis, followed by an ERK phosphorylation cascade. Acts as a receptor for osteocalcin (OCN) to regulate oligodendrocyte differentiation and central nervous system myelination. Mechanistically, plays a negative role in oligodendrocyte differentiation and myelination during development via activation of the ERK1/2 signaling pathway.

1. Imai, Y. et al. (2001) Cell 105, 891-902. PMID: 11439185
2. Meyer, RC. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 9529-34. PMID: 23690594
3. Berger, BS. et al. (2017) EMBO reports 18, 712-725. PMID: 28341812
Nota For research use only.
Imágenes
  • Anti-Human GPR37 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-GPR37 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Mouse brain lysate
    Lane 4: Rat brain lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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