中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HB747014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human GSPT1 Polyclonal Antibody (HB747014) is a rabbit polyclonal antibody detecting GSPT1 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
|
||||||||
| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human GSPT1 (Lys71-Asp499). | ||||||||
| ターゲット | Eukaryotic peptide chain release factor subunit 3a, GSPT1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, G1 to S phase transition protein 1 homolog, eRF3a, ERF3A | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P15170 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1) is a ~55 kDa protein. GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes. 1. Chauvin, C. et al. (2005) Molecular and cellular biology 25, 5801-11. PMID: 15987998 2. Cheng, Z. et al. (2009) Genes & development 23, 1106-18. PMID: 19417105 3. Hoshino, S. et al. (1989) The EMBO journal 8, 3807-14. PMID: 2511002 4. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242 5. Alkalaeva, EZ. et al. (2006) Cell 125, 1125-36. PMID: 16777602 6. Feng, T. et al. (2014) Molecular cell 53, 645-54. PMID: 24486019 7. Wang, X. et al. (2019) Cell 176, 625-635.e14. PMID: 30682371 | ||||||||
| 注意事項 | For research use only. |
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
