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| カタログ番号 | HY375015 |
|---|---|
| 説明 |
Anti-Human GZMA Monoclonal Antibody (1A612) [1A612] (HY375015) is a mouse monoclonal antibody detecting GZMA in ELISA, IHC, WB. Suitable for Human.
Highlights
|
| 種反応性 | Human |
| アプリケーション | ELISA, IHC, WB |
| 宿主種 | Mouse |
| クローン性 | Monoclonal |
| アイソタイプ | IgG |
| クローンID | 1A612 |
| 免疫原 | E. coli - derived recombinant human GZMA (Ile29-Val262). |
| ターゲット | Granzyme A, EC:3.4.21.78, CTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor, H factor, HF, GZMA |
| エンドトキシンレベル | < 10 EU/mg |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P12544 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 背景 | Granzyme A (GZMA) is a ~28 kDa protein. Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-B (GSDMB), releasing the pore-forming moiety of GSDMB, thereby triggering pyroptosis and target cell death. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA. 1. Hink-Schauer, C. et al. (2003) Nature structural biology 10, 535-40. PMID: 12819770 2. Zhou, Z. et al. (2020) Science (New York, N.Y.) 368. PMID: 32299851 3. Gershenfeld, HK. et al. (1988) Proceedings of the National Academy of Sciences of the United States of America 85, 1184-8. PMID: 3257574 4. Hameed, A. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3142-7. PMID: 3262682 5. Krähenbühl, O. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3471-7. PMID: 3263427 6. Hansen, JM. et al. (2021) Cell 184, 3178-3191.e18. PMID: 34022140 7. Gong, W. et al. (2022) Genes & diseases 9, 1405-1407. PMID: 36157507 8. Oltra, SS. et al. (2023) Cell death and differentiation 30, 1366-1381. PMID: 36899106 |
| 注意事項 | For research use only. |
SDS-PAGE for Anti-Human GZMA Monoclonal Antibody (1A612)
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