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Обзор
Каталожный номерHY202014
Описание
Anti-Human H2AB1 Polyclonal Antibody (HY202014) is a rabbit polyclonal antibody detecting H2AB1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human H2AB1 (Met1-Asp115).
Цель Histone H2A-Bbd type 1, H2A Barr body-deficient, H2A.B, H2A.Bbd, H2AB1, H2AFB1
Очистка Purified by antigen affinity column.
Номер доступа P0C5Y9
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Histone H2A-Bbd type 1 (H2AB1) is a ~12 kDa protein. Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase.

1. Tolstorukov, MY. et al. (2012) Molecular cell 47, 596-607. PMID: 22795134
2. Bao, Y. et al. (2004) The EMBO journal 23, 3314-24. PMID: 15257289
3. Okuwaki, M. et al. (2005) Molecular and cellular biology 25, 10639-51. PMID: 16287874
4. Doyen, CM. et al. (2006) The EMBO journal 25, 4234-44. PMID: 16957777
5. Menoni, H. et al. (2007) Molecular and cellular biology 27, 5949-56. PMID: 17591702
6. Eirín-López, JM. et al. (2008) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22, 316-26. PMID: 17726088
7. González-Romero, R. et al. (2008) Gene 413, 1-7. PMID: 18329190
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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