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| カタログ番号 | HW371014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human HADHA Polyclonal Antibody (HW371014) is a rabbit polyclonal antibody detecting HADHA in ELISA, IHC, WB. Suitable for Human, Mouse, and Pig.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human HADHA (Gly31-Gln763). | ||||||||
| ターゲット | 78 kDa gastrin-binding protein, HADH, HADHA, Trifunctional enzyme subunit alpha, mitochondrial, TP-alpha, Monolysocardiolipin acyltransferase | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P40939 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Trifunctional enzyme subunit alpha, mitochondrial (HADHA) is a ~83 kDa protein. Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA described here carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities while the trifunctional enzyme subunit beta/HADHB bears the 3-ketoacyl-CoA thiolase activity. Independently of subunit beta, HADHA also exhibits a cardiolipin acyltransferase activity that participates in cardiolipin remodeling; cardiolipin is a major mitochondrial membrane phospholipid. 1. Carpenter, K. et al. (1992) Biochemical and biophysical research communications 183, 443-8. PMID: 1550553 2. Liang, K. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 7039-7044. PMID: 29915090 3. Xia, C. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 6069-6074. PMID: 30850536 4. Kamijo, T. et al. (1994) Biochemical and biophysical research communications 199, 818-25. PMID: 8135828 5. Miklas, JW. et al. (2019) Nature communications 10, 4671. PMID: 31604922 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-HADHA polyclonal antibody at 1μg/ml on various samples.
Lane 1: THP-1 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: Mouse liver lysate
Lane 5: Mouse kidney lysate
Lane 6: Mouse heart lysate
Lane 7: Rat liver lysate
Lane 8: Rat kidney lysate
Lane 9: Rat heart lysate
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