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Anti-Human HINT1 Polyclonal Antibody (HW557014)

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Anti-Human HINT1 Polyclonal Antibody
Anti-Human HINT1 Polyclonal Antibody
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Vista general
Número de catálogoHW557014
Descripción
Anti-Human HINT1 Polyclonal Antibody (HW557014) is a rabbit polyclonal antibody detecting HINT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Pongo abelii, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human HINT1 (Met1-Gly126).
Objetivo Histidine triad nucleotide-binding protein 1, HINT, Adenosine 5'-monophosphoramidase, HINT1, PKCI-1, PRKCNH1, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1, PKCI1
Purificación Purified by antigen affinity column.
Número de acceso P49773
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Adenosine 5'-monophosphoramidase HINT1 is a ~13 kDa protein. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide.

1. Chou, TF. et al. (2005) The Journal of biological chemistry 280, 15356-61. PMID: 15703176
2. Weiske, J. et al. (2006) The Journal of biological chemistry 281, 27356-66. PMID: 16835243
3. Chou, TF. et al. (2007) Molecular pharmaceutics 4, 208-17. PMID: 17217311
4. Chou, TF. et al. (2007) The Journal of biological chemistry 282, 15137-47. PMID: 17337452
5. Wang, J. et al. (2012) The journal of physical chemistry. B 116, 6798-805. PMID: 22329685
6. Zhou, X. et al. (2013) Biochemistry 52, 3588-600. PMID: 23614568
7. Shah, R. et al. (2017) Biochemistry 56, 3559-3570. PMID: 28691797
8. Shah, RM. et al. (2018) Journal of molecular biology 430, 2709-2721. PMID: 29787766
9. Strom, A. et al. (2020) FEBS letters 594, 1497-1505. PMID: 31990367
Nota For research use only.
Imágenes
  • Anti-Human HINT1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-HINT1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Raji cell lysate
    Lane 2: K562 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Hela cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: MCF-7 cell lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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